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Dendrite pruning6/12/2023 ![]() Published by The Company of Biologists Ltd. Thus, this study reveals a JNK-independent role of Raw in regulating the secretory pathway and thereby promoting dendrite pruning.Īxon Dendrite JNK signaling Neuroglian Pruning Raw. Mechanistically, Raw facilitates Nrg downregulation and dendrite pruning in part through regulation of the secretory pathway. Moreover, Raw is required to modulate the secretory pathway by regulating the integrity of secretory organelles and efficient protein secretion. Importantly, we show that Raw promotes endocytosis and downregulation of the conserved L1-type cell-adhesion molecule Neuroglian (Nrg) prior to dendrite pruning. Raw appears to regulate dendrite pruning via a novel mechanism, which is independent of JNK signaling. Drosophila ddaC sensory neurons selectively prune their larval dendrites to sculpt the. Here, we identify an important and cell-autonomous role of the membrane protein Raw in dendrite pruning of ddaC neurons. Neuronal pruning is essential for proper wiring of the nervous systems in invertebrates and vertebrates. However, the molecular mechanisms underlying ddaC dendrite pruning remain elusive. Our results indicate that pruning is regulated in a homeostatic fashion to oppose excessive branching and promote a similar dendrite structure in DGCs. Drosophila ddaC sensory neurons selectively prune their larval dendrites to sculpt the nervous system during early metamorphosis. This indicates that the temporal specificity of dendrite pruning is dictated. The dendrite-specific pruning events involve the formation of swellings and retracting bulbs, resembling the axon/dendrite degenerative process associated with brain injury and neurodegenerative diseases. Mechanistically, I reported Orbit regulates both microtubule orientation and pruning of ddaC dendrites via suppressing Par-1 kinase.Neuronal pruning is essential for proper wiring of the nervous systems in invertebrates and vertebrates. dendrites following their glomeruli occupation, as was recently. In the second part, I focused on Orbit/CLASP which was isolated in a forward genetic screen as a novel regulator of dendrite pruning. Utilizing both genetic and pharmacological approaches, I suggested a causal relationship between microtubule disassembly and dendrite pruning. Here, we show that both the Ik2 kinase and Katanin p60-like 1 (Kat-60L1) of the Katanin family of microtubule severing proteins are required for dendrite severing. Little is known about the molecular mechanisms underlying dendrite severing. In the first part of my thesis, I reported 2 microtubule disassembly factors that are required for dendrite pruning of ddaC neurons: Exchange factor for Arf6 (Efa6) and Stathmin (Stai). Dendrite pruning involves dendrite severing, followed with debris removal. In Drosophila PNS, the ddaC neurons which specifically prune away their dendrites during metamorphosis, is an important model system to unravel the regulatory mechanisms of neuronal pruning. Neuronal Dendritic Pruning Dendritic Prunings Pruning, Dendritic Prunings, Dendritic Dendrite Pruning Dendrite Prunings Pruning, Dendrite. Neuronal pruning, a process to remove unnecessary dendrites/axons, is an essential developmental step of the nervous system as its dysregulation highly correlates with various neurological disorders. A SYSTEMATIC STUDY OF MICROTUBULE REGULATORS IN DENDRITE PRUNING OF DROSOPHILA DDAC NEURONS. A SYSTEMATIC STUDY OF MICROTUBULE REGULATORS IN DENDRITE PRUNING OF DROSOPHILA DDAC NEURONSĭendrite pruning, Exchange factor for Arf6, Stathmin, microtubule disassembly, Orbit/CLASP, microtubule orientationīU SHUFENG ().
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